If the National Anthem or Colors is played while in formation, what should a Marine do on the first note?

When the National Anthem or Colors is played while in formation, the proper protocol requires that Marines present arms as a mark of respect and honor. The action of saluting is specifically designated to occur on the order "Present Arms," which is a command used to instruct Marines to render the proper hand salute as a gesture of military courtesy.

In this context, acknowledging the National Anthem or Colors with a salute only on the command emphasizes the structured nature of military ceremonies and the importance of following established procedures. It keeps uniformity and discipline in ceremonial practices, ensuring that all Marines respond in a synchronized manner.

This differentiates the act of saluting from standing at attention or merely facing the source of music, which, while respectful actions, do not embody the full ceremonial significance of rendering honors as dictated by military custom.